in fusion cloning strategy (TaKaRa)
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in fusion cloning strategy
In Fusion Cloning Strategy, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1539 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fusion+cloning+strategy/In-Fusion+HD+Cloning+Plus/bio_rxiv__64898__2026__03__30__715387-228-18-21
Average 99 stars, based on 1539 article reviews
In Fusion Cloning Strategy, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1539 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fusion+cloning+strategy/In-Fusion+HD+Cloning+Plus/bio_rxiv__64898__2026__03__30__715387-228-18-21
Average 99 stars, based on 1539 article reviews
in fusion cloning strategy - by Bioz Stars,
2026-09
99/100 stars
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Construct:Article Title: Genetic Analysis of DEFECTIVE KERNEL1 Loop Function in Three-Dimensional Body Patterning in Physcomitrella patens Article Snippet: .. To make the corresponding At and Zm Loop complementation constructs, the Article Title: Campylobacter jejuni Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells Article Snippet: .. The final constructs were generated using the Article Title: CD147: a small molecule transporter ancillary protein at the crossroad of multiple hallmarks of cancer and metabolic reprogramming Article Snippet: Point mutations and deletion constructs were generated with Quick Change Site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer's description. .. For CD147-ECD construct residues corresponding to a signal peptide (amino acids 1-21 of human CD147) followed by FLAG tag, 6xHis affinity tag, a TEV cleavage site, and amino acids 22-2-5 of human CD147, were cloned into a mammalian expression vector (pCEP4, Invitrogen) between NdeI and BamH1 sites using In Cloning:Article Title: Genetic Analysis of DEFECTIVE KERNEL1 Loop Function in Three-Dimensional Body Patterning in Physcomitrella patens Article Snippet: .. To make the corresponding At and Zm Loop complementation constructs, the Article Title: Campylobacter jejuni Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells Article Snippet: .. The final constructs were generated using the Article Title: 1-Octen-3-ol – the attractant that repels Article Snippet: .. The Article Title: Genome-Wide Identification and Analysis of the Valine-Glutamine Motif-Containing Gene Family in Brassica napus and Functional Characterization of BnMKS1 in Response to Leptosphaeria maculans Article Snippet: The firststrand cDNA was synthesized from 1 μg total RNA using the first cDNA synthesis Kit (Thermo Scientific, Waltham, MA, USA). .. The Article Title: Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cells Article Snippet: All plasmid sequences are listed in Table S1; the original CD3ζHDRT and the original TRAC-HDRT are available via Addgene (CD3ζ -truncCARgsg: Addgene ID 215759, TRAC-Cas12a: 215769). www.moleculartherapy.org Molecular Therapy: Nucleic Acids Vol. .. 36 June 2025 11 Article Title: Overexpression of <scp><i>BnNAC19</i></scp> in <i>Brassica napus</i> enhances resistance to <i>Leptosphaeria maculans</i>, the blackleg pathogen of canola Article Snippet: .. The PCR- amplified BnNAC19 cDNA fragment was ligated to the pRI201- AN overexpression vector harbouring a CaMV 35S promoter using an In- Article Title: CD147: a small molecule transporter ancillary protein at the crossroad of multiple hallmarks of cancer and metabolic reprogramming Article Snippet: Point mutations and deletion constructs were generated with Quick Change Site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer's description. .. For CD147-ECD construct residues corresponding to a signal peptide (amino acids 1-21 of human CD147) followed by FLAG tag, 6xHis affinity tag, a TEV cleavage site, and amino acids 22-2-5 of human CD147, were cloned into a mammalian expression vector (pCEP4, Invitrogen) between NdeI and BamH1 sites using In Generated:Article Title: Campylobacter jejuni Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells Article Snippet: .. The final constructs were generated using the Over Expression:Article Title: Genome-Wide Identification and Analysis of the Valine-Glutamine Motif-Containing Gene Family in Brassica napus and Functional Characterization of BnMKS1 in Response to Leptosphaeria maculans Article Snippet: The firststrand cDNA was synthesized from 1 μg total RNA using the first cDNA synthesis Kit (Thermo Scientific, Waltham, MA, USA). .. The Article Title: Overexpression of <scp><i>BnNAC19</i></scp> in <i>Brassica napus</i> enhances resistance to <i>Leptosphaeria maculans</i>, the blackleg pathogen of canola Article Snippet: .. The PCR- amplified BnNAC19 cDNA fragment was ligated to the pRI201- AN overexpression vector harbouring a CaMV 35S promoter using an In- Polymerase Chain Reaction:Article Title: Overexpression of <scp><i>BnNAC19</i></scp> in <i>Brassica napus</i> enhances resistance to <i>Leptosphaeria maculans</i>, the blackleg pathogen of canola Article Snippet: .. The PCR- amplified BnNAC19 cDNA fragment was ligated to the pRI201- AN overexpression vector harbouring a CaMV 35S promoter using an In- Amplification:Article Title: Overexpression of <scp><i>BnNAC19</i></scp> in <i>Brassica napus</i> enhances resistance to <i>Leptosphaeria maculans</i>, the blackleg pathogen of canola Article Snippet: .. The PCR- amplified BnNAC19 cDNA fragment was ligated to the pRI201- AN overexpression vector harbouring a CaMV 35S promoter using an In- Plasmid Preparation:Article Title: Overexpression of <scp><i>BnNAC19</i></scp> in <i>Brassica napus</i> enhances resistance to <i>Leptosphaeria maculans</i>, the blackleg pathogen of canola Article Snippet: .. The PCR- amplified BnNAC19 cDNA fragment was ligated to the pRI201- AN overexpression vector harbouring a CaMV 35S promoter using an In- Article Title: CD147: a small molecule transporter ancillary protein at the crossroad of multiple hallmarks of cancer and metabolic reprogramming Article Snippet: Point mutations and deletion constructs were generated with Quick Change Site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer's description. .. For CD147-ECD construct residues corresponding to a signal peptide (amino acids 1-21 of human CD147) followed by FLAG tag, 6xHis affinity tag, a TEV cleavage site, and amino acids 22-2-5 of human CD147, were cloned into a mammalian expression vector (pCEP4, Invitrogen) between NdeI and BamH1 sites using In FLAG-tag:Article Title: CD147: a small molecule transporter ancillary protein at the crossroad of multiple hallmarks of cancer and metabolic reprogramming Article Snippet: Point mutations and deletion constructs were generated with Quick Change Site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer's description. .. For CD147-ECD construct residues corresponding to a signal peptide (amino acids 1-21 of human CD147) followed by FLAG tag, 6xHis affinity tag, a TEV cleavage site, and amino acids 22-2-5 of human CD147, were cloned into a mammalian expression vector (pCEP4, Invitrogen) between NdeI and BamH1 sites using In Clone Assay:Article Title: CD147: a small molecule transporter ancillary protein at the crossroad of multiple hallmarks of cancer and metabolic reprogramming Article Snippet: Point mutations and deletion constructs were generated with Quick Change Site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer's description. .. For CD147-ECD construct residues corresponding to a signal peptide (amino acids 1-21 of human CD147) followed by FLAG tag, 6xHis affinity tag, a TEV cleavage site, and amino acids 22-2-5 of human CD147, were cloned into a mammalian expression vector (pCEP4, Invitrogen) between NdeI and BamH1 sites using In Expressing:Article Title: CD147: a small molecule transporter ancillary protein at the crossroad of multiple hallmarks of cancer and metabolic reprogramming Article Snippet: Point mutations and deletion constructs were generated with Quick Change Site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) according to manufacturer's description. .. For CD147-ECD construct residues corresponding to a signal peptide (amino acids 1-21 of human CD147) followed by FLAG tag, 6xHis affinity tag, a TEV cleavage site, and amino acids 22-2-5 of human CD147, were cloned into a mammalian expression vector (pCEP4, Invitrogen) between NdeI and BamH1 sites using In |